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KMID : 1024420090130010064
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Food Engineering Progress
2009 Volume.13 No. 1 p.64 ~ p.69
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Distribution and Biodiversity of Lactic Acid Bacteria Having Bacteriocin-Like Activity from Fresh Fruits and Vegetables
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Park Young-Seo
Chung Myong-Soo
Sim Gun-Sub
Jang Jae-Kweon
Park Hoon
Choi Young-Jin
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Abstract
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From the 25 fresh fruits and vegetable products, 1,250 isolates grown on MRS agar media were screened for the inhibitory activity on the growth of Escherichia coli 0157:H7, Listeria monocytogenes, and Bacillus cereus as well as Lactobacillus plantarum, L. casei, and Lactococcus lactis subsp. lactis. Among them, 607 isolates (49% of total isolates) from 23 different foods produced growth inhibitory activity on the E. coli 0157:H7, L. monocytogenes, or B. cereus. When these isolates were screened for the inhibitory activity on the growth of L. plantarum, L. casei, and Lactococcus lactis subsp., 24 isolates (3% of total isolates) from 7 food samples showed bacteriocin-like activity. These isolates had typical physiological characteristics of lactic acid bacteria, which indicated these isolates were strains of lactic acid bacteria. The inhibitor from 3 out of 24 revealed as nicin. From the RAPD-PCR profiles, 24 strains was classified and it was also indicated that most of the strains isolated from same produce showed similar phylogenetic profile.
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KEYWORD
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Lactic acid bacteria, bacteriocin-Like activity, fruits and vegetables, biodiversity
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